Summer Training Program
Practical Training in Microbiology
Theoretical knowledge in Microbiology is not sufficient to
practice Microbiology. One must be proficient in handling microbiological
techniques. There is no use being good in performing tests
with kits supplied by companies. One becomes “kittologist” rather
than Microbiologist. By becoming a kittologist one becomes
only a repeater-becomes a blind performer of steps. One can
not device one’s own things and find solution to a problem
Plenty of problems arise in every microbiological situations
where new microbes spring up surprises every now and then in
human and animal health, industrial and other situations. Individuals
must handle all types of microbes and identify them. Practical
training is provided by eminent and experienced persons in
the field with necessary background theory. Personal attention
will be provided.
Students from Tamilnadu Universities can get the benefit.
Contact Us:
Dr.
T Sundararaj, MSc., Ph.D
Director JASMN Research & Education Foundation
Address: No.5,
I Cross Street,
Thirumalai Nagar, Perungudi,
Chennai-600096, India.
Web: http://www.jasmnfoundation.com
Email:jasmnfoundationj@gmail.com
Phone: 91-9444777855
SUMMER
TRAINING: HANDS ON TRAINING:
MICROBIOOGY, BIOTECHNOLOGY,
MOLECULARBIOLOGY
(TESTS
WHICH WILL BE USEFUL IN FUTURE PROJECT WORK)
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MODULE 1
GENERAL ASPECTS
- STERILIZATION OF MATERIALS (with principle and procedures)
- Moist heat
- Dry heat
- Filtration
- Quality control
- PREPARATION OF MEDIA (With principles and procedure
- Basal medium
- Enriched medium
- Selective medium
- Enrichment medium
- BACTERIAL GROWTH- ISOLATION AND PLATING TECHNIQUES
- Various methods of streaking and isolation
- DETERMINATION OF TOTAL VIABLE COUNT
- Pour plate technique
- Miles and Mishra’s method
- Surface plating method
- GROWTH KINETICS STUDIES
- Determination of optical density
- BACTERIAL IDENTIFICATION – BIOCHEMICAL
TESTS
- DEMONSTRATION OF VARIOUS ENZYME ACTIVITIES
- ANTIBIOTIC SUSCESPTIBILITY TESTING
- MIC DETERMINATION
- ESBL, METALO PROTEASES DETECTION
- PLASMID ISOLATION
- PLASMID DEMONSTRATION – GEL
ELECTROPHORESIS
- Techniques: 1-12, 4 weeks
- Techniques: 1-5 10 days
- Techniques : 1-5,8,9 15 days
- Techniques: 8-12 15 days
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MODULE 2
CLINICAL BACTERIOLOGY
- Sterilization techniques
- Preparation of media
- Staining techniques
- Antibiotic susceptibility testing – disc
- MIC determination
- ESBL DETECTION
- PROCESSING OF URINE AND REPORTING
- PROCESSING OF SPUTUM AND REPORTING
- PROCESSING OF VAGINAL SWAB AND REPORTING
- PROCESSING OF THROAT SWAB AND REPORTING
- PROCESSING OF WOUND SWAB /PUS AND REPORTING
- BLOOD CULTURE TECHNIQUES
- PROCESSING OF BODY FLUIDS AND REPORTING
- PROCESSING OF STOOL SAMPLE AND REPORTING
- Techniques: 1-14: 8 weeks
- Techniques: 1-8 :4 weeks
- Techniques: 5-8 15 days
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MODULE 3
MOLECULAR BIOLOGY TECHNIQUES
- STERILIZATION OF MATERIALS
- PREPARATIONOF MEDIA AND ISOLATION TECHNIQUES
- VIABLE COUNT
- GROWTH KINETICS
- ISOLATION OF PLASMIDS
- GELELECTROPHORESIS TECHNIQUES
- DNA ISOLATION AND DEMONSTRATION
- PCR TECHNIQUES
- Techniques: 1-8, 4 weeks
- Techniques: 5-8, 15 days
- Techniques: 7-8, 10 days
- Techniques : 1-4, 10 days
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MODULE 4
IMMUNOLOGICAL TECHNIQUES-BASICS
- Agglutination tests
- Haemagglutination test – blood grouping
- Bacterial slide agglutination test
- Widal test
- a. Principle, test and interpretation
- Brucella agglutination test
- Principle, test and interpretation
- VDRL/RPR test
- Principle, test and interpretation
- TPHA test
- Principle, test and interpretation
- ELISA test
- Principle, test and interpretation
- Techniques : 1-6, 15 days
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MODULE 5
IMMUNOLOGICAL TECHNIQUES-ADVANCED PREPARATION OF POLYCLONAL
ANTIBODY
- Preparation of an antigen for
- Preparation of adjuvants
- PREPARATION OF POLYCLONAL ANTIBODY
- Testing of antibody production
Techniques: 1-4: 6 weeks 7500/-
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MODULE 6
MINI PROJECT FOR BIOTECHNOLOGY
- PLACKETT-BURMAN DESIGN AND TESTING
10-15 DAYS
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MODULE 7
FOOD MICROBIOLOGY
- TOTAL PLATE COUNT(BACTERIA)
- COUNTS OF YEAST AND MOLD
- ENUMERATION, ISOLATION AND IDENTIFICATION OF E.coli
- ENUMERATION, ISOLATION AND IDENTIFICATION OF SALMONELLA
- ENUMERATION, ISOLATION AND IDENTIFICATION OF SHIGELLA
- ENUMERATION, ISOLATION AND IDENTIFICATION OF VIBRIO
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MODULE 8
ROUTINE CLINICAL BIOCHEMISTRY TRAINING |
- The fee for training varies with different modules
and components selected
- For details contact: 9444117141
- Training is available throughout
the year
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